Table 3.
Summary of variants in canine factor VIII coding sequence of normal and hemophilia A dogs
| Exon number |
|||||||||||||||||
| 1 | 7 | 7 | 8 | 10 | 12 | 12 | 12 | 14 | 14 | 14 | 14 | 14 | 14 | 15 | 16 | 25 | |
| Nucleotide number in reference sequence (NM_001003212) | 141 | 877 | 909 | 1094 | 1440 | 1774 | 1786 | 1807 | 2782 | 2868 | 2943 | 3608 | 4278 | 5086 | 5292 | 5490 | 6866 |
| Predicted normal dog coding sequence (XM_005640988.2) from dog X chromosome genomic sequence (NW_003726126.1) | c | a | a | a | t | t | c | a | a | t | g | c | a | a | c | g | g |
| mRNA from kidney and spleen (NM_001003212) and liver (reference 24) of normal dog | t | g | g | g | a | c | c | g | g | c | a | t | g | g | t | a | a |
| EST DN369010.1 (nucleotides 5013–5653) | – | - | - | - | - | - | - | - | - | - | - | - | - | a | t | g | - |
| Male hemophilia A dog (current study) | c | a | a | a | t | t | t | a | a | t | g | c | a | a | c | g | g |
| Heterozygous normal female dog (current study) | c/t | a | a | a | t | t | c | a | a/g | t | g/a | c/t | a | a | c/t | g | g |
| Amino acid change | syn | R>G | syn | N>G | N>K | F>L | R>X | N>D | S>G | syn | syn | P>L | syn | N>D | syn | syn | G>D |
syn, synonymous