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The relative catalytic efficiency (log Ω) for T7 RNA polymerase structures with and without the constrained hydrogen bonda.
| exp | normal | H bond forced | |
|---|---|---|---|
|
|
|||
| Wild Type | −3.3 | 1.1 | −3.2 |
| Y639F | −0.9 | −2.3 | −1.4 |
with 15kcal/mol hydrogen bond constraint between O2′ and the hydroxyl group in Y416 at 1.9 Å was imposed in the wild type while a corresponding distance constraint at 2.8 Å between O2′ and Hζ in F416 was imposed in the mutant
