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. 2016 Nov;90(5):1037–1044. doi: 10.1016/j.kint.2016.06.022

Figure 3.

Figure 3

B7-1 is not induced in murine models of podocyte injury. (a) The albumin-creatinine ratio in the different models of podocytes injuries. (b) Glomerular morphology in sham-operated mice, in mice injected with lipopolysaccharide (LPS) (day 1 post injection), in mice injected with Adriamycin (day 7 post injection), in NZB/W F1 mice (age 46 weeks), and in mice 2 months after nephrectomy (Nx). (c) Transmission electron microscopy of the glomerular basement membrane in kidneys of vehicle- or LPS-treated mice 1 day after injection. Bar = 0.5 μm. (d) Coimmunostaining for nestin (green) and 3 different anti-B7-1 antibodies (red, 2A2: ab86473 from Abcam; AF140: from R&D Systems; and H-208: SC-9091 from Santa Cruz Biotechnology Inc.) in the paraffin-embedded kidneys of all models of podocyte injuries. (e) Coimmunostaining for nestin (green) and anti-B7-1 antibody (red, AF140: from R&D Systems) in the frozen sections of kidney of all models of podocyte injuries. Bar = 10 μm. Analysis of variance followed by a Tukey-Kramer test; all models versus sham: *** P < 0.001. DAPI, 4′,6-diamidino-2-phenylindole.