Fig. 2.
Viability of human cancer cell lines after incubation with γδ T cells and L-ZOL. Cells were treated with ZOL or L-ZOL for 24 h at concentrations between 3 and 10 μM. The treatments were then removed and replaced with 2 × 105 γδ T cells for an additional 24 h, before an MTT assay was performed to determine residual tumour cell viability. The ZOL and L-ZOL were used at non-toxic concentrations, in the absence of γδ T cells. No background toxicity was found for γδ T cells without N-BP. However, a dose dependent toxicity was found in cells pre-treated with ZOL or L-ZOL (ZOL > L-ZOL), except for PANC0403. Data was expressed as mean ± SD (n = 5). *p < 0.05, (Student's t-test vs. naive).