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. 2016 Nov;90(5):1056–1070. doi: 10.1016/j.kint.2016.06.032

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© 2016 International Society of Nephrology.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S7 — Representative images showing sheep IgG immunoreactivity in glomeruli of Tmsb4x^+/y (A) and Tmsb4x^−/y (B) mice injected with nephrotoxic serum (NTS). (C) Quantification of mean fluorescence in 30 glomeruli per sample (Tmsb4^+/y + NTS, n = 9; Tmsb4x^−/y + NTS, n = 6). Plasma titers of murine IgG subclasses (D, IgG1; E, IgG2a; F, IgG2b; G, IgG3) against sheep IgG in control, not immunized Tmsb4x^+/y mice (n = 5) and in Tmsb4x^+/y (n = 9) and Tmsb4x^−/y (n = 6) mice 21 days after NTS injection. Data are presented as mean ± SEM. Bar = 20 μm, *P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.