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. 2016 Oct 21;7:1654. doi: 10.3389/fmicb.2016.01654

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Copyright © 2016 Venkatesan, Palaniyandi, Sharma, Bisht and Narayanan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rv2159c cloning, expression and purification. (A,B) SDS-PAGE (12% gel) separation of proteins and western immunoloblot with anti-GST antibody of E. coli BL21 cells expressed Rv2159c protein. Lane 1 shows the resolution of cytosolic proteins of BL21 cells (negative control); lane 2, BL21 cells expressed pDVA 2159-GST plasmid; lane 3 shows the purified recombinant GST-Rv2159c protein using glutathione sepharose affinity chromatography and lane M, protein molecular weight ladder.