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Catalytic activity of Rv2159c and its mutants. Alkyl hydroperoxidase activity of Rv2159c variants (WT, C81S, C84S and C81,84S) were detected by measuring acetophenone using HPLC. The peroxidase activity of Rv2159c was also checked in the presence and absence of its substrate, PknI. No catalytic activity was observed with non-specific protein (Ffh), PknI alone and buffer control. One way ANOVA was performed. *** Indicates P < 0.001.
