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2D gel electrophoresis for S-Rv2159c and As-Rv2159c strains. The cytoplasmic cell extracts of S-Rv2159c, As-Rv2159c and Rv constructs in M. tuberculosis were subjected to 2D gel electrophoresis. The CBB R250 stained gels (A) Rv, (B) S-Rv2159c, and (C) As-Rv2159c were shown. The arrow marks represents the difference in spots using PDQuest software were analyzed by mass spectrometry.
