Table 1.
Identification of the differentially regulated 2D protein spots of Rv, S-Rv2159c, and As-Rv2159c.
| Spot no. | Gene name | Accession no. | Mascot score | No. of matched peptides | Gene index | Theoretical pI/kDa | Expression |
|---|---|---|---|---|---|---|---|
| GROUP 1: INTERMEDIARY METABOLISM, RESPIRATION | |||||||
| 4 | Uncharacterised oxidoreductase, GuaB1 | Rv1843c | 69 | 1 | gi 492067680 | 6.29/49.93 | Absent in AsRv2159c |
| 8 | ATP-dependent CLP protease protelytic subunit 2, ClpP2 | Rv2460c | 74 | 4 | gi 15609597 | 4.75/23.5 | Absent in As-Rv2159c |
| 9, 10, 11 | Malate dehydrogenase, Mdh | Rv1240 | 496 | 4 | gi 489501402 | 4.39/34.28 | More in S-Rv2159c |
| 12 | Glycogen accumulation regulator, GarA | Rv1827 | 457 | 3 | gi 240104237 | 4.06/17.21 | More in S-Rv2159c |
| GROUP 2: VIRULENCE, DETOXIFICATION AND ADAPTATION | |||||||
| 16 | Universal stress protein family protein, TB31.7 | Rv2623 | 800 | 6 | gi 685981944 | 5.55/31.65 | Present in AsRv2159c |
| 18 | Iron-regulated universal stress protein family protein, TB15.3 | Rv1636 | 243 | 3 | gi 669108828 | 5.62/15.31 | More in AsRv2159c |
| 20, 21 | Heat shock protein, HspX | Rv2031c | 327 | 3 | gi 801196443 | 4.75/16.22 | Present only in AsRv2159c |
| GROUP 3: LIPID METABOLISM | |||||||
| 7 | Fatty acid CoA synthetase, FaD17 | Rv3506 | 79 | 2 | gi 764899599 | 4.93/53.73 | Less in As-Rv2159c |
| 14 | 3-hydroxyacyl-ACP dehydratase subunit, HadB | Rv0636 | 83 | 1 | gi 686051919 | 6.52/14.93 | More in S-Rv2159c |
| GROUP 4: CELL WALL AND CELL PROCESSES | |||||||
| 15 | Conserved protein with FHA domain, FhaA | Rv0020c | 399 | 3 | gi 15607162 | 4.65/56.88 | More in AsRv2159c |
| GROUP 5: INFORMATION PATHWAY | |||||||
| 6 | Ribonuclease HII protein, RnhB | Rv2902c | 64 | 3 | gi 686045826 | 10.47/27.64 | Absent in AsRv2159c |