Table 1.
Means ± SEM of four parameters of autofluorescent LF granules in sections of two muscles of 8-week-old normal and mdx mice
| Parameter | LF granules in normal | LF granules in mdx | Student’s t test | |||||
|---|---|---|---|---|---|---|---|---|
| (a) Diaphragm (n = 11) | (b) EDL (n = 10) | (c) Diaphragm (n = 8) | (d) EDL (n = 10) | a versus b | c versus d | a versus c | b versus d | |
| Granule size (µm2) | 4.83 ± 0.33 | 4.31 ± 0.42 | 9.85 ± 0.55 | 7.72 ± 0.38 | ns | ** | **** | **** |
| Feret diameter (μm) | 3.56 ± 0.13 | 3.57 ± 0.17 | 4.66 ± 0.10 | 4.10 ± 0.10 | ns | ** | **** | * |
| Number of granules/mm3 tissue | 1310 ± 270 | 1260 ± 270 | 64,900 ± 4600 | 9,320 ± 760 | ns | **** | **** | **** |
| Area fraction of granules (%) | 0.00464 ± 0.00117 | 0.00406 ± 0.00098 | 0.456 ± 0.052 | 0.0508 ± 0.0051 | ns | **** | **** | **** |
n = number of muscle samples
Significances of differences P: **** P < 0.0001; *** 0.0001 ≤ P < 0.001; ** 0.001 ≤ P < 0.01; * 0.01 ≤ P < 0.05; ns not significant