Table. Primer sequences for three nested reverse transcription-polymerase chain reaction genotyping assays.
| Nested assay set number | Primer name | Primer sequence (5’–3’) | PCR product size | Nucleotides targeted |
|---|---|---|---|---|
| 1 | RV8633F | AGCGACGCGGCCTGCTGGGG | 945 | 8,731–9,469 |
| RV9577R | CGCCCAGGTCTGCCGGGTCTC | |||
| RV8669F | GTGATGAGCGTGTTCGCCCTT | 873 | ||
| RV9541R | GTGTGTGCCATACACCACGCC | |||
| 2 | RV8812F | CAACACGCCGCACGGACAAC | 766 | 8,869–9,469 |
| RV9577R | CGCCCAGGTCTGCCGGGTCTC | |||
| RV8823F | ACGGACAACTCGAGGTCC | 727 | ||
| RV9541R | GTGTGTGCCATACACCACGCC | |||
| 3 | RV8669F-2B | GTGATGAGCGTGTTCGCCCT | 328 | 8,731–8,869 |
| RV8996R | CCACGAGCCGCGAACAGTCG | |||
| RV8691F-2B | CTAGCTACGTCCAGCACCC | 271 | ||
| RV8961R | CAAACCGGGGAGGCCCA |
PCR: polymerase chain reaction.