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. 2016 Oct 21;3(5):ENEURO.0130-16.2016. doi: 10.1523/ENEURO.0130-16.2016

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Copyright © 2016 Chenaux et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — SynDIG1 limits early-phase structural plasticity in large spines. A, Representative images illustrating small stimulated spines (arrowheads) on EGFP-expressing neurons from SynDIG1 ^β-gal homozygous mutant neurons (top) and neurons from WT mice (bottom). Time stamps are in minutes relative to LTP stimulation. Small spines are defined as <85% of average neighbor brightness. Scale bar, 1 µm. B, Time course of small spine structural plasticity. There are no significance differences between genotypes (WT, n = 14 stimulated target spines on 14 cells; SynDIG1 ^β-gal homozygous mutant, n = 13 stimulated target spines on 13 cells; p > 0.2 for all comparisons). Error bars indicate ±SEM. C, The average early (<15 min after stimulation) and late (>15 min after stimulation) change in small spine brightness by genotype. There are no significant differences between genotypes (p > 0.4 for all comparisons). Error bars represent ±SEM. D, Representative images illustrating large stimulated target spines (arrowheads) on EGFP-expressing neurons from SynDIG1 ^β-gal homozygous mutant neurons (top) and WT neurons (bottom). Time stamps are in minutes relative to LTP stimulation. Large spines are defined as >85% of average neighbor brightness. Scale bar, 1 µm. E, Time course of large spine structural plasticity. Large stimulated spines on WT neurons did not enlarge after stimulation (black line; n = 8 spines on 8 cells). Large stimulated spines on SynDIG1 ^β-gal homozygous mutant neurons enlarged significantly 3 and 12 min after stimulation (gray line; n = 7 spines on 7 cells; p < 0.05). Error bars represent the SEM. F, The average early (<15 min after stimulation) and late (>15 min after stimulation) change in large spine brightness by genotype. Large stimulated spines on SynDIG1 ^β-gal homozygous mutant neurons enlarged significantly more than those of WT neurons during early-phase, but not late-phase, structural plasticity (p < 0.05). Error bars represent ±SEM.