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. 2016 Oct 21;6:35889. doi: 10.1038/srep35889

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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In Vitro Experiments (a) Perl’s Prussian blue stain identifies iron labelled cells in blue (scale bar x 100). (b) JIMT1BR3-Fluc/GFP + cells seeded at various concentrations (c) A strong linear correlation is seen between cell number and BLI signal; R² = 0.928 (d) MPIO labeled JIMT1BR3-Fluc/GFP + cells (L) and non labeled JIMT1BR3-Fluc/GFP + cells (R). (e) There was no significant difference in BLI signal detected in cells that were labeled with MPIO and cells that were not labeled (ns, p value = 0.82). These results are representative of three experiments with three wells of each condition.