Figure 5. Pathways through which oxidative stress may regulate miR-34a expression.

BEAS2B cells were treated with either PIK75 (at 10 μM) or vehicle (DMSO) for 1 hour prior to stimulation with or without 100 μM H₂O₂ for 48 hours, RNA extracted and (A) SIRT1 or (B) SIRT6 expression examined (n = 6). BEAS2B cells were treated with either (C) PIK75, (D) AS-605240, (E) IC-87114 and (F) GSK2636771 (10 μM) or vehicle (DMSO) for 1 hour prior to stimulation with or without 100 μM H₂O₂ for 48 hours, RNA extracted and miR-34a levels assessed (n = 3–5). BEAS-2B cells were transfected with small interfering RNA (siRNA) against either PTEN for 24 h or a random oligonucleotide control and then either left un-stimulated or stimulated with 100 μM H₂O₂ for 48 hours (n = 4). RNA was extracted and either (G) PTEN, (H) SIRT1, (I) SIRT6 or (J) miR-34a levels assessed. Data are means ± SEM analyzed by Mann-Whitney and Kruskal–Wallis test with post hoc Dunns ^#P < 0.05, *P < 0.05, ***P < 0.001.