Table 3. Sequences of primers used in IPCR.
Primer | Sequence | Reference |
---|---|---|
sul1 inv FWa | AAGAACCGCACAATCTCGTC | 42 |
sul1 inv RVa | GGCTTCCGCTATTGGTCTC | 42 |
sul1 inv nest 1FWb | CGAAGAAGGAGTCCTCGGTG | This study |
sul1 inv nest 2FWb | GACTCGCAGCATTTCGATCG | This study |
sul1 inv nest 12RVb,c | TCGGAAACCCTCGCGAAATT | This study |
sul1 inv nest 3FWb | CGACATCCACGACGTCTGAT | This study |
sul1 inv nest 3RVb | GCCAGAGACCGAGGGTTAGA | This study |
tetM inv FWa | CAGAATTGTTAGAGCCATATC | 23 |
tetM inv RVa | GCAGAAATCAGTAGAATTGC | 23 |
asul1 inv FW and RV, and tetM inv FW and RV primers have been translated in to reverse complement from previously published primers
bPrimers used in nested PCR have the word nest attached to the primer name
csul1 inv nest 12RV was used with both sul1 inv nest 1FW and 2FW primers as the reverse primer.