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. 2016 Oct 21;6:35751. doi: 10.1038/srep35751

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Copyright © 2016, The Author(s)

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(A) RNA gel blot detection of mature PSTVd RNA, GFP mRNA, PSTVd-derived siRNA (siPSTVd) and GFP-derived siRNA (siGFP) accumulation in 16c plants co-infiltrated with 35S-GFP and either 35S-GUS control or 35S-PSTVd at 4 dpi. Methylene blue-stained ribosome rRNA and U6 hybridization were used as the loading control. (B) Observation of local GFP silencing in the leaves of 16c plants co-infiltrated with 35S-GFP and either 35S-GUS control or 35S-PSTVd. Photographs were taken under UV light at 2, 3 and 4 dpi. (C) Observation of systemic GFP silencing spread to the whole plant induced by co-infiltrated 35S-GFP with either 35S-GUS control or 35S-PSTVd in separate plants. Photographs were taken under UV light at 4, 5 and 8 dpi.