Skip to main content
. 2016 Oct 21;6:35751. doi: 10.1038/srep35751

Figure 2. Effects of PSTVd-induced host Virp1 on the acceleration of GFP silencing in transient assays.

Figure 2

(A) GFP fluorescence in the leaves of 16c plants co-infiltrated with 35S-GFP and either 35S- GUS control or 35S-PSTVd or 35S-∆PSTVd. Photographs were taken under UV light at 2 dpi. (B) RNA gel blot detection of Virp1 mRNA and mature PSTVd RNA accumulation in 16c plants and Virp1 silencing leaves co-infiltrated with 35S-GFP and either 35S-GUS control or 35S-PSTVd or 35S-∆PSTVd at 4 dpi. Methylene blue-stained ribosome rRNA was used as the loading control. (C) GFP fluorescence in leaves of 16c plants co-infiltrated with 35S-GFP and either 35S-GUS, 35S-P19 control or 35S-PSTVd or 35S-Virp1. Photographs were taken under UV light at 4 dpi.