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. 2016 Oct 21;6:35751. doi: 10.1038/srep35751

Figure 3. Effects of PSTVd replication and Virp1 on the expression and DNA methylation of the GFP transgene.

Figure 3

(A) GFP fluorescence in leaves of 16c plants co-infiltrated with 35S-GFP and either 35S-GUS control, 35S-PSTVd or 35S-Virp1. Photographs were taken under UV light at 4 dpi. (B) RNA gel blot detection of GFP mRNA, mature PSTVd RNA and siGFP accumulation from samples as described in (A). Methylene blue-stained ribosome rRNA and U6 hybridization were used as the loading control. (C) Percentage of CG, CHG, and CHH methylation of the 35S promoter of the 35S-GFP transgene by bisulfite sequencing analysis from samples as described in (A). The original data are shown in the Supplementary Figure S4. The statistical analysis was performed using the OriginPro 8 program.