. 2016 Oct 21;6:35716. doi: 10.1038/srep35716

Table 1. Kinetic parameters of S385Y/D469T/R520Q towards 3-FBA, 4-FBA and 3-HBA and improvement over the previous variant.

Mutant	Substrate	K_M (mM)	Fold improvement	k_cat (s⁻¹)	Fold improvement	k_cat/K_M (s⁻¹M⁻¹)	Fold improvement
D469T	3-FBA	56 (10)	N.A.	13.2 (1.5)	N.A.	240 (50)	N.A.
	4-FBA	251 (240)	N.A.	5.0 (4.4)	N.A.	20 (26)	N.A.
	3-HBA	N.A.	N.A.	N.A.	N.A.	N.A.	N.A.
D469T/R520Q	3-FBA	13 (4)	4.3	6.0 (0.75)	0.5	470 (170)	2.0
	4-FBA	25 (8)	10.0	0.20 (0.03)	0.0	8.1 (2.8)	0.4
	3-HBA	N.A.	N.A.	N.A.	N.A.	N.A.	N.A.
S385Y/D469T/R520Q	3-FBA	1.3 (0.4)	10.0	7.0 (0.3)	1.2	5400 (1490)	11.5
	4-FBA	15 (3)	1.7	1.7 (0.1)	8.5	110 (21)	13.6
	3-HBA	390 (10)	N.A.	2.1 (0.2)	N.A.	5.4 (0.1)	N.A.

Standard deviations are shown in brackets. (Data from Payongsri¹⁵).