Table 1.
List of mutants screened in the initial round of screening in an intramammary sheep infection model
| Group I-15 mutants | Group II-17 mutants | Group III-19 mutants | |||
|---|---|---|---|---|---|
| Mutant | Genes disrupted | Mutant | Genes disrupted | Mutant | Genes disrupted |
| Apo7 | Hypothetical Protein (MAG1890) | Apo2-12 | Hypothetical protein, predicted lipoprotein (MAG1050) | 3-20 | NADH oxidase (nox) |
| Apo6 | Sugar isomerase (araD) | 1-3-0 | Hypothetical protein (MAG2810) | 13 | VpmaX |
| 26 | Oligopeptide transporter ATP binding protein (oppF) | 15-2 | Hypothetical protein, predicted lipoprotein (MAG6200) | 14 | DNA methylase (MAG1790) |
| 3-15 | Elongation factor G (fusA) | 170 | Hypothetical protein (MAG0110) | 137 | Hypothetical protein (MAG4460) |
| 6-9-2 | Oligopeptide ABC transporter permease (oppC) | 3-29 | Hypothetical protein/Vpma like predicted lipoprotein (MAG2540) | 8-1 | Deoxyguaosine kinase (dgk) |
| 6-14 | Hexose P transport protein (uhpT) | 4-1-0 | GTP-dependent nucleic acid-binding protein (engD) | 1-2-0 | Pentitol posphotransferase enzyme II (MAG6360) |
| 6-21 | Cell division protein (mraZ) | 8 + 1-4 | ABC transporter ATP-binding protein (MAG5960) | 96-2 | ATP synthase (atpA) |
| 6-29 | Pyruvate dehydrogenase beta subunit (pdhB) | 8 + 1-8 | Transport protein SGAT (ulaA) | 5-1 | Hypothetical protein (MAG0250) |
| 6-32 | Phosphate acetyl transferase (eutD) | 3-4-0 | Hypothetical protein (MAG3390) | 6-13 | Hypothetical protein (MAG1570) |
| 7 + 1-8 | Hypothetical protein (MAG4010) | 9-28 | Conserved hypothetical protein (MAG0390) | 7 + 1-3 | Conserved hypothetical protein (MAG3650) |
| 9-40 | Glycerol ABC transporter permease component (gtsB) | 62 | Lipoate Protein Ligase A (lplA) | 23 | Aminopeptidase (MAG5520) |
| 3-1 |
Phosphoenol-
pyruvate phosphotransferase ( ptsL ) |
3-1 | Phosphoenolpyruvate Phosphotransferase ( ptsL ) | 3-1 | Phosphoenolpyruvate Phosphotransferase ( ptsL ) |
| 8 + 1-5 | Ribonuclease H II ( rnhB ) | 8 + 1-5 | Ribonuclease H II ( rnhB ) | ||
| 9-31 | Alcohol dehydrogenase (adhT) and NADH oxidase (nox) | 9-31 | Alcohol dehydrogenase (adhT) and NADH oxidase (nox) | ||
| 6-15 | Esterase/lipase ( lip ) | 6-15 | Esterase/lipase ( lip ) | ||
| 81-1 | Oligopeptide ABC transporter permease (oppB) | 81-1 | Oligopeptide ABC transporter permease (oppB) | ||
| 9-8 | Dihydrolipoamide dehydrogenase (pdhD) | 6-12 | Transcriptional regulator (MAG6310) | ||
| 212 | Type III restriction modification system (MAG1530) | San5 | Predicted lipoprotein (MAG3120) | ||
| 9-16 | Conserved hypothetical protein (MAG1450) | 3-23 | Lipoprotein (MAG6410) | ||
| 6-15 | VpmaY | ||||
Mutants kept common (“watermarks”) between the groups are underlined and those shortlisted for secondary screening showing >95% absence are in italics.