Fig. 1.

Overview on the production process of (A) denovoDerm and (B) denovoSkin. A A skin biopsy is removed from the patient and dermis and epidermis are separated. Fibroblasts are isolated and incorporated in a collagen type I hydrogel. The dermal substitute denovoDerm is transplanted onto the wound bed and covered with split-thickness skin. B For denovoSkin, a skin biopsy is removed from the patient and dermis and epidermis are separated. Fibroblasts and keratinocytes are isolated. Fibroblasts are incorporated in a collagen type I hydrogel. After a cultivation phase, keratinocytes are seeded on top of this hydrogel. The dermo-epidermal skin substitute denovoSkin is transplanted onto the wound bed and does not require additional covering due to the epidermal cell layer on top of the hydrogel.