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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Nat Immunol. 2016 May;17(5):471. doi: 10.1038/ni.3411

Figure 1.

Figure 1

Mice can inherit Vα2 but not recognize SIINFEKL. (a) Flow cytometry analyzing binding of the H-2Kb–SIINFEKL tetramer by CD45.2+B220–CD8+ cells in blood from a ‘true’ OT-I mouse. (b) Flow cytometry analyzing Vα2 staining on CD8+ cells in blood from a mouse with partial loss of the OT-I transgene. (c) Flow cytometry as in a of cells from the mouse in b. Numbers adjacent to outlined areas indicate percent tetramer-positive CD8+ cells (a,c) or CD8+Vα2+ cells (b). Cells were stained with antibody to CD45.2 (anti-CD45.2) (104; BioLegend), anti-CD8a (56-7.8; BioLegend), anti-B220 (RA3-6B2; BioLegend) and anti-Vα2 (B20.1; eBioscience). The H-2Kb–SIINFEKL tetramer was made in baculovirus as described8.