Fig. 3.

Chromosomal positions of mean DNA methylation differences. a Modified Manhattan plot of mean DNA differences by chromosomal position along the x-axis and mean DNA methylation differences on the y-axis. Each point represents a CpG site with mean DNA methylation differences between 2 and 10 years (black) and between 10 and 16 years (gray) for CpGs and mean DNA methylation differences between 2 and 10 years (red) and between 10 and 16 years (green) at the aDMPs (n = 26). b Scatter plot of DNA methylation at CpGs situated in a CpG island overlapping the promoter and the first exon of SOCS3 generated by RRBS (upper panel) and EpiTYPER (replication data set, bottom panel) on the y-axis and chromosomal position on the x-axis. Each point represents a CpG per sample colored by age (red = 2, blue = 10, and green = 16 years) sorted by chromosomal position, and each point is jittered by 0.1). Smoothening lines represents local regression colored by time points. The significant aDMP (chr17: 76355061) is highlighted between vertical lines