Table 3.
Results of four different algorithms for SPF layer-type chicken tissue samples of healthy birds or those infected with Histomonas meleagridis
| Healthy SPF chickens | Infected with H. meleagridis | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Genes | Delta CT | BestKeeper© | NormFinder | GeNorm | RefFinder | Delta CT | BestKeeper© | NormFinder | GeNorm | RefFinder |
| RPL13 | 2.49 | 0.73 | 0.745 | 1.302 | 1 | 1.58 | 0.7 | 0.509 | 0.753 | 2 |
| TFRC | 2.61 | 1.24 | 1.192 | 1.302 | 2 | 1.64 | 0.88 | 0.719 | 0.848 | 3 |
| TBP | 2.52 | 1.32 | 0.978 | 1.364 | 3 | 1.52 | 0.82 | 0.377 | 0.753 | 1 |
| VIM | 2.86 | 1.3 | 1.406 | 1.963 | 4 | 2.21 | 1.37 | 1.733 | 1.402 | 5 |
| RPS7 | 3.14 | 2.12 | 2.377 | 1.626 | 5 | 1.87 | 1.04 | 1.132 | 1.112 | 4 |
| HMBS | 3.6 | 2.27 | 2.862 | 2.31 | 6 | 2.41 | 1.43 | 1.984 | 1.837 | 6 |
| HPRT1 | 3.73 | 2.52 | 2.944 | 2.666 | 7 | 2.4 | 1.92 | 2 | 1.634 | 7 |
| RPLP0 | 4.96 | 2.88 | 4.588 | 3.239 | 8 | 2.61 | 1.79 | 2.261 | 2.03 | 8 |
Delta CT compares CT value from two reference genes; BestKeeper© calculates standard deviations (SD) based on raw crossing point; NormFinder gives stability value (Sv) by comparing inter and intra group variations; GeNorm value represents the average expression stability (M); RefFinder compares all other algorithms and gives an overall ranking on the basis of geometric mean.