Fig. 3.

Induction of C3 expression after KA injection in neurons and glial cells in the CA3 region of the hippocampus of adult rats. a Double fluorescent immunohistochemistry showing C3 expression (green) near hippocampal neurons labeled with neurotrace (red). b Representative images showing the expression of C3 (green) and microglial cells stained with tomato lectin (red). c Immunohistochemistry performed using a fluorescent antibody against GFAP (green) together with a DAB-stained anti-C3. Scale bar, 20 μm. Inset scale bar, 10 μm. DAPI was used as a nuclear marker. All images represent the maximum intensity projection, and orthogonal views are also shown, generated by projecting z-series in the x- and y-planes. d Double-immunofluorescence images showing C/EBPβ expression after KA injection in neuronal cells (NeuN-positive cells). Scale bar, 50 μm