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. 2016 Aug 18;22(42):14940–14949. doi: 10.1002/chem.201602697

Figure 2.

Figure 2

The reaction of 25 mm oxazolone 1 with 5 mm 5’‐AMP in 100 mm MES buffer (pH 6.5) at room temperature; (a) HPLC analysis of the medium (after a 50‐fold dilution of a 20 μL sample of the reaction medium in the buffer) after 5 h of reaction using method A and detection at 248 nm (brown) and 273 nm (blue); (b) evolution of the amount of the presumed mixed anhydride diastereomers 5 (open circles) and 5’ (filled circles) and of the presumed esters 6 and 7 at the 2’‐ and 3’‐position (open squares).