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. 2016 Aug 18;22(42):14940–14949. doi: 10.1002/chem.201602697

Figure 4.

Figure 4

The reaction of 25 mm oxazolone 1 with 5 mm 5’‐dAMP in 100 mm MES aqueous buffer (pH 6.5) at room temperature; (a) HPLC analysis of the medium (after a 50‐fold dilution of samples of the reaction medium (20 μL) in the 100 mm MES buffer pH 6.5) after 7 h of reaction using method A, detection 248 nm (brown) and 273 nm (blue); (b) evolution of the amount of the presumed mixed anhydride diastereomers 9 (filled circles) and 9’ (open circles).