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The reaction of 25 mm 5(4 H)‐oxazolone (1) with 5 mm 3’‐AMP in a pH 6.5 MES buffer. (a) HPLC analysis of the reaction medium after 3.7 h using method A, detection 248 nm (brown) and 273 nm (blue); (b) evolution of the yield of adducts 10 (open squares, presumed to be the ester at the 2’‐position), 11’ (filled circles, putatively attributed to a mixed anhydride), and 12 (open diamonds, presumed bis‐adduct).
