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. 2016 Oct 21;11(10):e0165189. doi: 10.1371/journal.pone.0165189

Fig 1. Duloxetine inhibits function of recombinant P2X4R.

Fig 1

(A) Effects of duloxetine (3–30 μM) and PPADS (10 μM) on ATP (10 μM)-induced [Ca2+]i responses in rP2X4R-1321N1 cells measured by the FDSS 7000EX system. Each column indicates the value as a percentage of control (Ctl: 10 μM ATP alone-induced [Ca2+]i responses) (n = 4, ***P < 0.001 vs. control). (B) Effect of duloxetine at various concentrations (1–10 μM) on ATP (10 μM)-induced [Ca2+]i respoznses in hP2X4R-1321N1 cells measured at a single cell level. Each column shows the effect of duloxetine evaluated by the S2 (2nd [Ca2+]i response) / S1 (1st [Ca2+]i response) (n = 4, ***P < 0.001 vs. control). Cells were pretreated with duloxetine and PPADS for 10 min prior to application of ATP. Data represent mean ± SEM for all groups.