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. 2016 Oct 21;11(10):e0165189. doi: 10.1371/journal.pone.0165189

Fig 4. Effect of duloxetine on other P2 receptors.

Fig 4

(A) Effect of duloxetine at various concentrations (3–30 μM) and PPADS (10 μM) on BzATP (100 μM)-induced [Ca2+]i responses in rP2X7R-1321N1 cells measured by the FDSS 7000EX system. Each column shows the value as a percentage of control (100 μM BzATP alone-induced [Ca2+]i responses) (n = 4). (B, C) Effects of duloxetine (30 μM) on [Ca2+]i responses induced by BzATP (100 μM) (B) and ADP (10 μM) (C) measured by a single cell analysis in primary cultured rat microglial cells. Each column shows the relative increase ratio (F340/F380) from the basal level prior to BzATP or ADP application (n = 4, **P < 0.01). Cells were pretreated with duloxetine or PPADS for 10 min prior to application of BzATP or ADP. Data represent mean ± SEM for all groups.