Fig 6. Effect of the primary imatinib metabolite in humans, N-desmethyl imatinib, on inhibition of P. falciparum parasitemia.

P. falciparum strain Palo Alto cultures were treated during ring stage (0.5% parasitemia) with increasing concentrations of (A) imatinib or N-desmethyl imatinib, and % parasitemia of infected cultures was determined by flow cytometry every 24 hours after treatment to monitor parasite replication. Note, open markers inhibited all parasitemia. (B) To create a dose response curve, cultures were treated with concentrations of N-desmethyl imatinib, and the % inhibition of parasitemia was determined by flow cytometry after a 60 h incubation.