TABLE 1 .
Multiple reaction monitoring parameters of peptides (calibrator and internal standard) selected for targeted analysis of human hepatobiliary transporters
The labeled amino acid residue of the internal standard is shown in bold and italic.
| Transporter | Signature Peptides | Parent Ion | Product Ions |
Cone (V) | CE (V) | RT | On-Column Calibration Rangea | |
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | |||||||
| z = 2 | z = 1 | z = 1 | min | femtomole | ||||
| BCRP | SSLLDVLAAR | 523.0 | 757.4 | 644.3 | 40 | 18 | 21.0 | 2.76–88 |
| SSLLDVLAAR | 527.8 | 654.5 | 767.5 | |||||
| BSEP | STALQLIQR | 515.2 | 529.1 | 657.1 | 32 | 18 | 15.4 | 2.92–93 |
| STALQLIQR | 520.3 | 539.4 | 667.4 | |||||
| MATE1 | GGPEATLEVR | 514.7 | 816.9 | 688.0 | 30 | 14 | 12.2 | 3.46–111 |
| GGPEATLEVR | 519.8 | 627.4 | 698.4 | |||||
| MRP2 | LTIIPQDPILFSGSLR | 885.4 | 989.1 | 1329.7 | 30 | 26 | 22.2 | 2.29–73 |
| LTIIPQDPILFSGSLR | 890.5 | 999.6 | 1339.7 | |||||
| MRP3 | ADGALTQEEK | 531.1 | 634.3 | 747.3 | 30 | 16 | 8.2 | 1.96–125 |
| ADGALTQEEK | 535.3 | 642.3 | 755.4 | |||||
| MRP4 | AEAAALTETAK | 538.2 | 733.0 | 875.4 | 30 | 17 | 9.8 | 6.41–103 |
| AEAAALTETAK | 542.3 | 741.4 | 883. 5 | |||||
| NTCP | GIYDGDLK | 440.7 | 547.1 | 710.1 | 30 | 13 | 12.5 | 3.42–219 |
| GIYDGDLK | 444.7 | 555.3 | 718.3 | |||||
| OATP1B1 | NVTGFFQSFK | 587.9 | 860.5 | 961.4 | 35 | 17 | 20.1 | 1.33–85 |
| NVTGFFQSFK | 591.9 | 868.5 | 969.5 | |||||
| OATP1B3 | IYNSVFFGR | 551.8 | 826.5 | 526.6 | 30 | 15 | 18.0 | 1.31–84 |
| IYNSVFFGR | 556.8 | 635.2 | 836.6 | |||||
| OATP2B1 | VLAVTDSPAR | 514.8 | 646.3 | 816.4 | 30 | 16 | 11.2 | 1.56–100 |
| VLAVTDSPAR | 519.9 | 656.3 | 826.4 | |||||
| OCT1 | LSPSFADLFR | 576.7 | 476.6 | 768.0 | 25 | 23 | 21.9 | 1.38–88 |
| LSPSFADLFR | 581.8 | 778.4 | 865.4 | |||||
| P-gp | NTTGALTTR | 467.6 | 618.3 | 719.4 | 30 | 16 | 8.4 | 1.50–96 |
| NTTGALTTR | 472.6 | 628.3 | 729.5 | |||||
CE, Collision energy; RT, retention time.
a
The low end of this range reflects the lower limit of quantification of the assay.