Skip to main content
. 2016 Oct 21;11(10):e0164896. doi: 10.1371/journal.pone.0164896

Fig 4. Srpr is a direct target of miR-330-5p in mouse keratinocyte.

Fig 4

(A) Location of the binding site for miR-330-5p in 3’UTR of Srpr mRNA. Deletion is depicted in red hyphen. (B) Dual Luciferase assay showed that miR-330-5p directly inhibited the luciferase activity by targeting the binding site in Srpr 3’UTR. However, luciferase activity did not change significantly when cells were co-transfected with the deletion mutant lacking the miR-330-5p-binding site and the miR-330-5p. (C-E) Mir-330-5p suppressed the proliferation of PAM212 cells through inhibition of SRPR expression. (C) Real-time quantitative PCR showed that cotransfection of miR-330-5p and the full length SRPR-cDNA (Srpr CDS) was able to abolish the decreased expression of Srpr by miR-330-5p alone. (D) Proliferation of PAM212 cells was decreased by miR-330-5p. Co-transfection with the Srpr CDS construct rescued inhibition of cell proliferation by miR-330-5p. (E) Relative viable cells were measured at 72 h after transfection. (B-E) Results are the average of three independent experiments. **P<0.01; ***P<0.001. NS = no significance.