Table 1. Mean age predictions of female and male wild-type Ae. aegypti mosquitoes using the cross validation method for samples used in the model and the prediction method for samples that were excluded from the model.

Wild-type Ae. aegypti females						Wild-type Ae. aegypti males
	Cross validation set1 [N = 101]		Prediction set2 [N = 501]			Cross validation set1 [N = 142]			Prediction set2 [N = 253]
Actual age	Mean predicted age [95% CI]	SEM	Actual age	Mean predicted age [95% CI]	SEM	Actual age	Mean predicted age [95% CI]	SEM	Actual age	Mean predicted age [95% CI]	SEM
1	3.0a[0.9–5.6]	0.9	1	5.3a [3.8–6.7]	0.7	1	5.7a[3.6–7.7]	0.9	9	8.5a [7.1–10.0]	0.7
5	6.5b[4.8–8.3]	0.8	5	4.1a [3.1–5.1]	0.5	5	7.2a,b[5.5–8.8]	0.7	13	10.4a [9.5–11.4]	0.5
17	15.9c[14.7–17.2]	0.5	9	10.7b [9.6–11.7]	0.5	9	8.2a,b[6.6–9.8]	0.7	17	16.8b [15.9–17.8]	0.5
21	18.3c[16.8–19.8]	0.7	13	14.6c [12.8–16.4]	0.9	17	15.2c[13.7–16.6]	0.7	21	19.5c [18.3–20.8]	0.6
			17	18.0d,f [16.8–19.3]	0.6	21	17.3c[15.9–18.7]	0.6	25	21.5c [20.5–22.6]	0.5
			21	19.6c,d,e,f [17.3–22.0]	0.9	30	28.1d[24.8–31.8]	1.6	30
			25	23.2e [22.1–24.2]	0.5
			30	19.9f[18.9–20.9]	0.3

Means followed by the same letter are not significantly different at P<0.05 when using Tukey post hoc test

Actual and mean predicted ages shown are in days

¹ The accuracy of samples used to develop calibration models

² The accuracy of samples used to validate models