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. 2016 Oct 21;11(10):e0164506. doi: 10.1371/journal.pone.0164506

Fig 1. Regulation of Talin1 and Kindlin3 expression by Gfi1b and LSD1.

Fig 1

A-B. Enrichment of Gfi1b and its co-factor LSD1 at two sites on the talin1 (Tln1p1 and Tln1p2) and one site (kind3p) on the kindlin3 promoter in undifferentiated (U; uninduced) (A) versus differentiated (I; induced) (B) L8057 cells (Mk line). C. Upregulation of Kindlin3 and Talin1 message in LSD1 inhibited primary megakaryocytes derived from in vitro culture of fetal liver cells (1° megs). D. Upregulation of Kindlin3 and Talin1 message in gfi1b-/- total fetal liver cells relative to wild type controls. E. Dose dependent repression of the isolated kindlin3 and talin1 promoters (sequence depicted in S1A and S1B Fig) by the indicated amounts of Gfi1b in HEK-293T cells. All graphs depict averages (solid bars) and standard deviations (error bars) from three independent experiments. P values are represented by various (*, §, +) symbols for different data series with values of < 0.05, 0.01 and 0.001 being indicated by one, two and three symbols, respectively.