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. 2016 Oct 21;11(10):e0164506. doi: 10.1371/journal.pone.0164506

Fig 2. Up-regulation of Talin1, Kindlin3 and integrin expression upon megakaryocyte differentiation.

Fig 2

A. Time course of Talin1, Kindlin3 and integrins GPIIb/CD41 and CD61 expression during in vitro megakaryocytic differentiation of total fetal liver cells. B. Gfi1b, Kindlin3, Talin1 and CD61 protein levels relative to β-actin (loading control) in freshly isolated total fetal liver cells (FL) versus megakaryocytes derived from them following in vitro culture (1° megs). 20–30 μg of total protein was loaded per lane. C. Up-regulation of kindlin3, talin1 and CD61 expression in the human hematopoietic cell line K562 uninduced (U) or induced to differentiate into megakaryocytes (I) with phorbol ester. D. Western blot of Gfi1b and corresponding proteins relative to β-actin. 60 μg of total protein was loaded per lane. All graphs depict averages (solid bars) and standard deviations (error bars) from three independent experiments. Western results of one from three representative experiments are shown. P values are represented by various (*, §, +) symbols for different data series with values of < 0.05, 0.01 and 0.001 being indicated by one, two and three symbols, respectively.