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. 2016 Oct 21;11(10):e0164506. doi: 10.1371/journal.pone.0164506

Fig 5. Effect of combined manipulation of Talin1 and Kindlin3 expression on megakaryocytic differentiation.

Fig 5

A. Kindlin3, talin1 and megakaryocytic marker message levels in control (Scr; scrambled) versus Talin1 and Kindlin3 knocked down fetal liver cells differentiated into megakaryocytes. B. Acetyl choline esterase staining of the corresponding cells. P values were <0.001. C. Message levels of indicated factors in control (pCDH; empty vector), Talin1 head domain (THD) and Kindlin3 and Talin1 full length (TFL) and kindlin3 over-expressing fetal liver cells differentiated into megakaryocytes. P values are represented by various (*, §, +) symbols for different data series with values of < 0.05, 0.01 and 0.001 being indicated by one, two and three symbols, respectively. E. Immunoprecipitation of kindlin3 and CD61 with Talin1. Input represents 10% of lysate used for the immuno-precipitation. D. Acetyl choline esterase staining of the corresponding cells. The mean ± s.d. of acetylcholine-positive cells as a percentage of the total population from three independent experiments is indicated. P values were <0.05.