Protein retention of RCCs. (A) Percent protein retention of eRCCs and sRCCs relative to protein content of intact cells, analyzed via BCA assay. (B) Coomassie stain of three separate eRCC and sRCC samples as compared to the parent cells (whole) from which they were derived. Arrowheads indicate bands that were present in whole parent cells and largely depleted by eRCCs but maintained by sRCCs. (C) Western blot of three separate eRCC and sRCC samples as compared to the parent cells (whole) from which they were derived, probing for proteins localized to various cellular compartments: TRP-2 (membrane-associated), β-actin (cytoplasmic), lamin B1 (nuclear envelope), and histone H3 (nuclear). Data represent the mean ± SD, *p<0.05.