Table 1. Characterization of mice subjected to aortic banding.
| Sham AB24h | AB24h | ShamAB1w | AB1w | ShamAB3w | AB3w | ShamAB16w | AB16w | Sham AB18w | AB18w | |
|---|---|---|---|---|---|---|---|---|---|---|
| Animal and organ weights | ||||||||||
| N | 10 | 10 | 10 | 9 | 10 | 9 | 10 | 9 | 5 | 8 |
| Body weight (g) | 25.0 ± 0.9 | 23.5 ± 0.4 | 26.8 ± 0.4 | 26.3 ± 0.5 | 26.4 ± 0.5 | 24.1 ± 0.6* | 34.4 ± 1.2 | 23.4 ± 1.2*** | 36.4 ± 2.4 | 25.9 ± 1.9** |
| LVW/TL (mg/mm) | 4.82 ± 0.17 | 5.84 ± 0.44* | 5.09 ± 0.06 | 8.25 ± 0.21*** | 5.24 ± 0.18 | 8.87 ± 0.19*** | 5.85 ± 0.10 | 12.41 ± 0.33*** | 5.93 ± 0.38 | 12.22 ± 0.16*** |
| LW/TL (mg/mm) | 8.51 ± 0.26 | 13.16 ± 1.07*** | 8.95 ± 0.20 | 20.01 ± 1.40*** | 8.99 ± 0.15 | 20.40 ± 1.53*** | 9.38 ± 0.11 | 23.87 ± 1.29*** | 9.61 ± 0.34 | 24.51 ± 1.51*** |
| M-mode echocardiography | ||||||||||
| N | 0 | 0 | 6 | 7 | 8 | 6 | 6 | 9 | 3 | 8 |
| LAD (mm) | NA | NA | 1.70 ± 0.02 | 3.02 ± 0.09*** | 1.81 ± 0.02 | 3.20 ± 0.13*** | 1.70 ± 0.09 | 3.49 ± 0.20*** | 1.80 ± 0.06 | 3.63 ± 0.19*** |
| LVPWd (mm) | NA | NA | 0.75 ± 0.03 | 1.08 ± 0.02*** | 0.75 ± 0.03 | 1.12 ± 0.02*** | 0.82 ± 0.05 | 1.08 ± 0.02*** | 0.82 ± 0.01 | 1.06 ± 0.04** |
| LVIDd (mm) | NA | NA | 4.08 ± 0.11 | 4.09 ± 0.05 | 4.27 ± 0.09 | 4.05 ± 0.12* | 4.02 ± 0.19 | 5.87 ± 0.12*** | 4.26 ± 0.05 | 5.75 ± 0.14*** |
| FS (%) | NA | NA | 18.87± 1.29 | 14.29 ± 1.22* | 20.94 ± 0.91 | 15.20 ± 1.68* | 18.09 ± 1.66 | 6.81 ± 0.84*** | 15.25 ± 1.66 | 6.65 ± 0.89*** |
| Left ventricular mRNA expression | ||||||||||
| N | 10 | 10 | 10 | 9 | 10 | 9 | 10 | 9 | 3 | 8 |
| NPPA/ RPL32 | 1.0 ± 0.5 | 3.2 ± 0.7* | 1.0 ± 0.1 | 11.0 ± 1.65*** | 1.0 ± 0.5 | 9.8 ± 0.7*** | 1.0 ± 0.1 | 48.4 ± 4.9*** | 1.0 ± 0.2 | 78.7 ± 16.3* |
| NPPB/ RPL32 | 1.0 ± 0.1 | 11.5 ± 1.5*** | 1.0 ± 0.1 | 3.0 ± 0.4*** | 1.0 ± 0.1 | 2.3 ± 0.2*** | 1.0 ± 0.1 | 6.2 ± 0.2*** | 1.0 ± 0.5 | 8.5 ± 0.4*** |
| ACTA1/ RPL32 | 1.0 ± 0.2 | 1.7 ± 0.4* | 1.0 ± 0.2 | 6.5 ± 1.3*** | 1.0 ± 0.2 | 7.0 ± 0.9*** | 1.0 ± 0.2 | 4.6 ± 1.3** | 1.0 ± 0.5 | 11.4 ± 2.2* |
Post-mortem organ weights, M-mode echocardiographic recordings and left ventricular mRNA expression data of male mice 24h, 1, 3, 16 and 18 weeks after aortic banding (AB), and of sham-operated controls for each time-point. Statistical differences were tested using an unpaired t-test vs. respective sham-operated controls
*p<0.05
**p<0.01
***p<0.001. Relative mRNA expression of the hypertrophic marker gene skeletal muscle α-actin (ACTA1), heart failure signature molecules atrial (NPPA/ANP) and brain (NPPB/BNP) natriuretic peptides normalized to ribosomal protein L32 (RPL32) expression. LVW, left ventricular weight; TL, tibia length; LW, lung weight; LAD, left atrial diameter; LVPWd, left ventricular posterior wall thickness in diastole; LVIDd, left ventricular internal diameter in diastole; FS, fractional shortening; NA, not available.