FIGURE 3.
Electrophoretic mobility gel shift assay of wild-type and genetically-altered glucocorticoid elements in the PLN promoter sequences. Electrophoretic mobility gel shift assays were used to determine DNA–protein complex formation using nuclear extracts from mouse hearts. NF-k B was used as a positive technical control (lanes1and 2); nonlabeled wild-type (PLN-WT, lanes 3 and 4) and altered (PLN-MT, lanes 5 and 6) were used as specific competitors; and nuclear lysate as a negative control (lanes 7–10); PLN-MT oligonucleotide (lanes11and12) and PLN-WT oligonucleotide (lanes13 and14). Duplicate samples were assayed for each treatment.