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. Author manuscript; available in PMC: 2016 Oct 21.
Published in final edited form as: Hum Mutat. 2008 May;29(5):640–647. doi: 10.1002/humu.20692

FIGURE 3.

FIGURE 3

Electrophoretic mobility gel shift assay of wild-type and genetically-altered glucocorticoid elements in the PLN promoter sequences. Electrophoretic mobility gel shift assays were used to determine DNA–protein complex formation using nuclear extracts from mouse hearts. NF-k B was used as a positive technical control (lanes1and 2); nonlabeled wild-type (PLN-WT, lanes 3 and 4) and altered (PLN-MT, lanes 5 and 6) were used as specific competitors; and nuclear lysate as a negative control (lanes 7–10); PLN-MT oligonucleotide (lanes11and12) and PLN-WT oligonucleotide (lanes13 and14). Duplicate samples were assayed for each treatment.

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