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. Author manuscript; available in PMC: 2016 Oct 21.
Published in final edited form as: Hum Mutat. 2008 May;29(5):640–647. doi: 10.1002/humu.20692

FIGURE 4.

FIGURE 4

Electrophoretic mobility gel shift assay of the PLN wild-type and genetically-altered glucocorticoid element specificity in the presence of common glucocorticoid consensus. Consensus glucocorticoid receptor element (GRE), PLN-WT, and PLN-MT motifs were used to examine binding specificity in cardiac nuclear extracts from wild-type mice. Lanes1–3: cardiac nuclear extracts reacted with radiolabeled oligonucleotides of PLN-WT, PLN-MT, and consensus GRE sequences. Lanes 4–6:consensus GRE oligonucleotides were used as specific competitor (100 × unlabeled GRE oligonucleotides). Lanes 7–9 and lanes 10–12: PLN-WT and PLN-MT oligonucleotides were used as competitors (100×-fold unlabeled oligonucleotide), respectively.