Figure 3.
Contractile parameters were measured in infected adult rat cardiomyocytes. A) Representative traces for contractility. B) Fractional shortening (FS %). C) Contracting velocity (+dL/dT). D) Relaxing velocity (−dL/dT). On stimulation with 100 nM isoproterenol (Iso), cardiomyocytes infected with control or WT inhibitor-1 viruses displayed enhanced contractility, demonstrated by increased fractional shortening, accelerated contraction and enhanced relaxation; #P < 0.05, +Iso vs. −Iso. However, the effect of isoproterenol was significantly blunted in cardiomyocytes with overexpression of G147D inhibitor-1; *P < 0.05, G147D vs. GFP or WT. n = 4 hearts/group; 20–40 cells/group. Values represent means ± SE.