Skip to main content
. Author manuscript; available in PMC: 2016 Oct 21.
Published in final edited form as: Lab Invest. 2010 Oct 18;91(3):439–451. doi: 10.1038/labinvest.2010.172

Figure 8.

Figure 8

Analysis of LX2 cell activation by TGFβ. (a) Quantitative real-time RT-PCR analysis of procollagen type 1, and α-smooth muscle actin mRNA levels normalized on GAPDH. The data represent the mean ±S.D. of duplicate experiments run in triplicates. (b) Determination of collagen 1 protein amount by dot blot in medium of LX2 cells with normal (scr) or silenced decorin content (siDcn) with or without TGFβ1 treatment. (c) Diagram of the relative decorin proteoglycan content from media of cells transfected with either scrambled or decorin-specific siRNAs. The data represent the mean ±SD of duplicate experiments run in triplicates; *P<0.05, **P<0.01.