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. 2016 Oct 21;11(10):e0165135. doi: 10.1371/journal.pone.0165135

Fig 2. Establishing a NheA specific sandwich EIA.

Fig 2

(A) Standard curve for the quantification of rNheA by the highly sensitive sandwich EIA in which mAb 1G4 served as capture and 2G11-HRP as detection antibody. The detection limit is as low as 20 pg/ml. (B) Exemplified reactivity of toxin containing supernatants from B. cereus strains in the NheA-specific sandwich EIA; MHI 1507 (dot); MHI 241 (square) and MHI 1308 (triangle up). The samples were assayed in duplicates and depicted as means and standard deviation (SD).