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. 2016 Oct 21;11(10):e0165135. doi: 10.1371/journal.pone.0165135

Fig 6. Immunofluorescence microscopy of NheA on Vero cells.

Fig 6

Staining of cell-bound NheA after treatment of Vero cells with B. cereus supernatants. (A) MHI 1507 NheB (100 ng ml-1 and approx. 10 ng ml-1 NheC) with mAb 1G4 neutralized NheA untreated Vero cells stained with primary and secondary antibody. (B) MHI 1761 (containing NheB at 100 ng ml-1 and approx. 10 ng ml-1 NheC) supplemented with neutralized rNheA (200 ng ml-1). (C) Stained Vero cells treated with rNheA only. (D) Untreated Vero cells stained with primary and secondary antibody. All slides were counterstained with DAPI.