Table 1. Overview on the most important characteristics of the three mAbs against NheA applied in the present study.

mAb	Ig subtype	sensitivity rNheA#	Inhibitions of antibody reactivity by soluble antigen	Titer* in indirect EIA (strain no.)
				MHI163	MHI165		MHI241	DSM31	NVH 1280/33
1G4	IgG2a	107 ng ml-1	63%	1:103 (± 12)	1:30 (± 10)	1:39 (± 5)		1:98 (± 13)		1:94 (± 17)
1A8	IgG1	negative	negativ	1:760 (± 13)	1:870 (± 1)	1:861 (± 3)		1:631 (± 15)		1:974 (± 3)
2G11	IgG2a	98 ng ml-1	42%	1:10 (± 3)	1:7 (± 5)	1:15 (± 10)		n. a		n. a

^# sensitivity defined as the concentration of rNheA resulting in an OD₄₅₀ of 0.3 under the conditions of an indirect EIA; values in brackets indicate the variation coefficient in %.

n. a. not analyzed

* dilution resulting in an OD₄₅₀ of 1.0