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. 2016 Oct 21;11(10):e0165135. doi: 10.1371/journal.pone.0165135

Table 2. Results of the mAb 1G4-based IAC purification of NheA from B. cereus MHI 241 supernatants.

Fraction NheA units1 (reciprocal EIA titer) Volume (ml) total units % NheA
sample 1,436 15 ml 21,542 100
flow through 80 15 ml 1212 5.62
wash 20 30 ml 600 2.8
eluate 1 1,576 10 ml 15,760 73.2
eluate 2 14 10 ml 143 0.66

1 determined by indirect EIA using mAb1A8