Table 4. Reactivity of the recombinant NheA fragments in different EIA set-ups substantiate the epitope-mapping experiments based on synthetic peptides and allow the allocation of the 1G4 and 2G11 epitope.

antibody	EIA set-up	antigen
		NheA FL	NheA-109	NheA-164	NheA-185
1G4	indirect	+	+	*	*
2G11	indirect	+	-	-	-
1G4/2G11-HRP*	sandwich	+	-	-	-

* remaining EIA reactivity approx. 10–20%