Transgene silence of OsNPR1-OX restored growth and development. A, Expression levels of OsNPR1 measured by qRT-PCR in wild-type TP309, G316, and two transgene-silenced G316 plants (S1 and S2) with the rice UBIQUITIN gene (Os03g13170) as an internal control, using primers listed in Supplemental Table S2. B and C, Decreased disease resistance of the transgene-silenced plants to Xoo (strain PXO99A) in comparison with TP309, G316, and the fully resistant Xa21 line 106 control (Song et al., 1995). D, Normal morphological phenotype of the transgene-silenced line (S1) compared with the wild type and G316 during the heading stage. E and F, Statistical analysis of plant heights (E) and seed-setting rates (F) of TP309, G316, S1, and S2 plants grown at the Shanghai station. Data were collected from adult plants grown in the field. Different letters indicate significant differences at P < 0.05 (n = 20; ANOVA followed by Duncan’s multiple range test).