Figure 2.

mat3 exhibits defects in the male gametophyte. A, Representative images of pollen from wild-type (WT) and mat3 plants after in vitro germination for 6 h. Bars = 100 μm. B, Pollen germination percentage at 6 h. Data are means ± se of three experiments. For each independent replicate, 400 to 500 pollen grains were analyzed. C, Pollen tube lengths of the wild type and mat3 after germination for 6 h. Data are means ± se of three experiments. D, Semi in vivo pollen tube growth. Shown are wild-type and mat3 homozygous pistils 12 h (left) and 24 h (right) after pollination stained with decolorized Aniline Blue. E, Whole-mount images of mature siliques after clearing in a 0.2 n NaOH and 1% SDS solution.